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Plant Transformation Methods

3.8
(17 votes)
8 questions
English
AI Generated
Test your knowledge on various plant transformation methods including Agrobacterium and In Planta techniques.
1
48 random squares
AI Generated

Which method uses a soil bacterium to transfer genes into plants?

Agrobacterium

Electroporation

Gene Gun

In Planta

56% got this right

Agrobacterium tumefaciens is commonly used for this method.
2
48 random squares
AI Generated

Which plant transformation method involves direct application to the plant?

In Planta

Agrobacterium

Electroporation

Gene Gun

46% got this right

In Planta methods are often simpler and do not require tissue culture.
3
AI Generated

In which present-day country was Agrobacterium tumefaciens first discovered?

Hard  0% got this right

Agrobacterium tumefaciens was first identified in the Netherlands in the early 20th century.
4
48 random squares
AI Generated

What is the approximate size (in kilobases) of the Ti plasmid used in Agrobacterium-mediated transformation?

150
200
213
kilobases

Hard  28% got this right

The Ti plasmid is a large plasmid, typically around 200 kilobases in size.
5
48 random squares
AI Generated

Which method is known for its simplicity and does not require tissue culture?

In Planta

Agrobacterium

Electroporation

Gene Gun

Hard  11% got this right

In Planta methods are often used for their simplicity and efficiency.
6
48 random squares
AI Generated

Select all methods that are used for plant transformation.

Agrobacterium

In Planta

Electroporation

Gene Gun

Hard  29% got this right

Agrobacterium and In Planta are two common methods for plant transformation.
7
48 random squares
AI Generated

Order the following steps in Agrobacterium-mediated transformation.

1.

Infection

2.

Integration

3.

Selection

4.

Regeneration

Easy  94% got this right

The typical steps are infection, integration, selection, and regeneration.
8
48 random squares
AI Generated

Which method involves the use of a gene gun to deliver DNA into plant cells?

Gene Gun

Agrobacterium

Electroporation

In Planta

56% got this right

The gene gun method uses high-velocity microprojectiles to deliver DNA.