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Test your knowledge on various plant transformation methods including Agrobacterium and In Planta techniques.
1
48 random squares
Which method uses a soil bacterium to transfer genes into plants?
Agrobacterium
Electroporation
Gene Gun
In Planta
54% got this right
Agrobacterium tumefaciens is commonly used for this method.
2
48 random squares
Which plant transformation method involves direct application to the plant?
In Planta
Agrobacterium
Electroporation
Gene Gun
42% got this right
In Planta methods are often simpler and do not require tissue culture.
3
In which present-day country was Agrobacterium tumefaciens first discovered?
0% got this right
Agrobacterium tumefaciens was first identified in the Netherlands in the early 20th century.
4
48 random squares
What is the approximate size (in kilobases) of the Ti plasmid used in Agrobacterium-mediated transformation?
150 —
200
— 213
kilobases
25% got this right
The Ti plasmid is a large plasmid, typically around 200 kilobases in size.
5
48 random squares
Which method is known for its simplicity and does not require tissue culture?
In Planta
Agrobacterium
Electroporation
Gene Gun
14% got this right
In Planta methods are often used for their simplicity and efficiency.
6
48 random squares
Select all methods that are used for plant transformation.
Agrobacterium
In Planta
Electroporation
Gene Gun
27% got this right
Agrobacterium and In Planta are two common methods for plant transformation.
7
48 random squares
Order the following steps in Agrobacterium-mediated transformation.
1.
Infection
2.
Integration
3.
Selection
4.
Regeneration
94% got this right
The typical steps are infection, integration, selection, and regeneration.
8
48 random squares
Which method involves the use of a gene gun to deliver DNA into plant cells?
Gene Gun
Agrobacterium
Electroporation
In Planta
55% got this right
The gene gun method uses high-velocity microprojectiles to deliver DNA.
